Royal Jelly (RJ) was studied for its efficiency as a potential alternative to Fetal Bovine Serum (FBS) in cell culture medium. An all natural cell culture medium was formulated by using tender coconut, Royal Jelly and Honey. This medium was studied in comparison with the commonly used DMEM and FBS medium. The results showed that although there was not any significant difference observed between the cell proliferations in the two different cell culture medium, the RJ medium exhibited a strong anti-microbial atmosphere for the cells to grow and proliferate. This study was aimed at promoting a serum- free cell culture medium for tissue culture application.