The ethanol extract of the root bark of the plant Securidaca longepedunculata Fresen (Polygalaceae) was found to be toxic against the albino mice at medium (600 mg/kg) and higher doses (1000 mg/kg) when administered orally through gavages. The LD50 calculated was found to be 547.72 mg/kg. The root bark did not show any significant hypoglycemic effect on Streptozotocin induced albino mice. Six heavy metals were analyzed but only three metals (Zn, Cu, Mn) could be detected in the root and root bark of this plant after microwave assisted digestion at optimized condition using Flame Atomic Absorption Spectroscopy (FAAS) technique. Out of these six, three metals (Cr, Pb and Cd) were found below the method detection limit and the rest (Zn, Mn and Cu) were within the permissible range according to specification made by the World Health Organization (WHO).