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Method development and validation of sitagliptin and metformin using reverse phase HPLC method in bulk and tablet dosage form | Abstract
Scholars Research Library

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Abstract

Method development and validation of sitagliptin and metformin using reverse phase HPLC method in bulk and tablet dosage form

Author(s): Karimulla S K, Vasanth P M*, Ramesh T, Ramesh M

A Simple, accurate and precise method for estimation of reversed phase HPLC method has been developed for the simultaneous determination of sitagliptin and Metformin by using Hypersil BDS C18 (100 x 4.6 mm, 5μm particle size) column and mobile phase of at 215 nm. A mobile phase has a composition of potassium dihydrogen orthophosphate and methanol(50:50v/v),adjusted the pH 8.5 with o-phosphoric acid was used and flow rate 1.0ml/min. Retention times of Sitaglipitin and Metformin were 2.3 min and 4.6 min respectively. The method developed validated as per ICH guideline


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