Heterocyclic molecules represent the most utilized scaffolds for the discovery of novel synthetic drugs. As reported in recent communications, the pyrrole moiety can be found both in natural and synthetic pharmaceutical products. Pyrroles have been reported to play an important role as antibacterial, antiviral and anti-inflammatory agent. An efficient synthesis by Microwave assisted process of pyrrole derivatives by the Paal-Knorr Condensation of benzoin with primary aromatic amines in refluxing ethanol resulted in the formation of α-amino ketone intermediates, which were condensed without isolation, with Malononitrile. Various derivatives such as 2-amino-4, 5-diphenylpyrrole-3-carbonitriles (1a-1d) were prepared. The synthesis of pyrrole derivatives are formed by direct reaction of a heterocyclic compound with substituted anilines using microwave irradiation. This optimized method was used for the synthesis of a series of four substituents of pyrrole derivatives. In-vitro anti-inflammatory activity of synthesized compounds was analyzed by using spectroscopic quantification by protein denaturation method. The synthesized compounds were confirmed through spectral characterization using IR and polymorphism study. The synthesized pyrrole derivatives show promising in vitro anti-inflammatory and antifungal activity.