The current research study performed was to evaluate the myocardial protective outcome of methanolic extract of Mucuna pruriens against isoproterenol prompted myocardial necrosis in rats. The rats stayed pretreated with methanolic extract of Mucuna pruriens at two dissimilar doses of 250mg/kg and 500 milligram/kg, correspondingly intended for thirty days. Myocardial necrosis was persuaded in the rats by administering isoproterenol (85mg/kg s.c) injection. The measures of serum cardiac marker enzymes such as creatine kinase myoglobin (CK-MB), lactate dehydrogenase (LDH), serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), total cholesterol (TC), triglycerides (TG), low density lipoproteins (LDL), very low density lipoproteins (VLDL), high density lipoproteins (HDL) and total protein (TP) were estimated. In addition, plasma TBARS and plasma LDH levels were also recorded. Antioxidant parameters like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione (GSH) were performed in heart tissue homogenate. The result of the current study specified that, methanol extract of Mucuna pruriens pre-co-treatment prohibited nearly entire parameters of isoproterenol persuaded myocardial necrosis in rats. The overhead outcome was established by the histopathological investigation. It can be recognized that methanolic extract of Mucuna pruriens has a substantial outcome on the protection of the heart against isoproterenol encouraged myocardial necrosis through sustaining endogenous antioxidant enzyme actions, which provides scientific proof of the traditional values.