Nutritional attributes of the fungal treated sugarcane bagasse which include the crude ash, crude fiber, crude fat, moisture, total carbohydrates and total energy value of the fungal treated sugarcane bagasse were probedin the study. This in in line with the previous study of Valentino et al 2016 wherein the crude protein content of sugarcane bagasse were enhanced by the same species of endophytic fungi after 20 days of solid state fermentation. Varying effect of the endophytes was noted on the moisture, crude ash, crude fiber and crude fat of the fermented sugarcane bagasse. Among which the following showed the highest increment; Fusarium sp. 2 and Penicilliumcitrinumtreated sugarcane bagasse of 9.10% for the moisture content; for the % ash, Aspergillusniger- treated sugarcane bagasse with 7.71% ;Aspergillusflavus- treated sugarcane bagasse of 1.3% for the crude fat while Monascusrubertreated sugarcane bagasse for the crude fiber with 56.03%. Meanwhile, decrease in total carbohydrates and total energy value were recorded. For the cytotoxicity assay, non cytotoxic effect of the single cell protein enriched sugarcane bagasse was observed.