Several causes of infertility have been identified all over the world. In Nigeria, infertility is a common reproductive health problem of great concern especially to the female population. Hormonal problems are amongst the important factors in the evaluation of female infertility. This study is mainly concerned with the pattern of the reproductive hormone level in infertile women in South Western Nigeria. The study involve a total of 100 subjects (50 infertile and 50 fertile women as control) and hormone assays were carried out to assess the hormone pattern in these women using ELISA technique. The infertile subjects were married for at least 2 years and the semen analysis of their husbands was normal. The anthropometric details (age, height and weight) of both the test subjects and controls were obtained. The hormonal levels of the infertile women was compared to control groups and a significantly lower LH, FSH, progesterone and estradiol levels (P=0.000, p=0.000, p=0.000 and p=0.005 respectively i.e. p < 0.05) and significantly higher prolactin level were observed in the infertile women (p = 0.012 i.e. p<0.05). Also, 14 out of 50 infertile patients (28%) had hyperprolactinemia, 40 out of 50 infertile (80%) had low progesterone, and 45 out of 50 infertile patients (90%) had low estradiol while all the 50 infertile patients had low gonadotrophins (LH and FSH) levels.