The hepatic and some serum enzymes activity of garlic, antioxidant vitamins C and E on pefloxacin-induced toxicity in wistar rat were evaluated. Method: One hundred adult wistar rats (120-180), of either sex were randomly selected into five study groups. Each group comprised of 10 pairs (ten males and ten females) were not allowed to mate, with group 1 as control. Group 11 were pefloxacin treated only while group 111 to 1V were pefloxacin treated with either garlic, vitamins C and E. Pefloxacin, garlic vitamin C and E in doses 11.43mg/kg, 4.28mg/kg, 14.29mg/kg body weight in normal saline (vehicle) was administered orally by intubation to both male and female of groups 11 to V for 14 days. Control animals received 0.5ml of normal saline. In life observation measurements were taken and at the end of drug, garlic, antioxidant vitamins C and E combined administration animals were sacrificed and tissues obtained for biochemical assessment. The effect of pefloxacin only, pefloxacin in diet with antioxidant vitamins C, E and garlic supplements respectively on the activity of serum enzyme aspartate aminotransaminase, alanine aminotransaminase, alkaline phosphotase, acid phosphotase, prostatic acid phosphotase, Pefloxacin administration induced a significant (P<0.05) increase in the activities of AST and ALT in male and female rats relative to the control. There was a significant (P<0.05) decrease in AST and ALT of all the antioxidant supplemented groups.