With the emerging worldwide interest in adopting and studying traditional systems of medication and exploiting their potential based on different health care systems, the evaluation of the rich heritage of traditional medicine is essential. From the innumerable plants being researched since time immemorial, Cressa cretica is an important one. To evaluate the diagnostic Pharmacognostical characters of Cressa cretica along with their physico-chemical parameters, fluorosence analysis and TLC profile. The Pharmacognostical characters were determined in terms of macroscopy, microscopy, powder microscopy, leaf constant, fluorescence analysis and preliminary phytochemical analysis. The findings of macroscopy revealed that leaves ovate or elliptic-to scale like, flowers solitary, axillary, sessile or on short peduncles, fruits capsular, ovoid, unilocular, Seed one seeded, glabrous and smooth & stem first erect and then become decumbent with horizontal Roots. Transverse section of stem depicted the single celled epidermis, hypodermises, cortex, parenchymatous cells, lignified xylem. Transverse section of root septate hairs, epidermis, arenchyma, starch grains, Cortex, spherical vesicles. Transverse section of leaf palisade cells, trichomes, paracytic and anomocytic stomata. Preliminary phytochemical screening exhibited the presence of various phytochemical groups like Alkaloids, Carbohydrate, Flavonoids, Fixed Oils & Fats, Glycosides, Steroids, Tannins & phenolic compound. Further the leaf constant, powder microscopy, fluorescence characterstics & TLC profile indicating results from this investigation. Various Pharmacognostical and physico-chemical parameters have pivotal roles in identification, authentication and establishment of quality parameters of the species.