Aim of the Study: To find the effect of omega-3 fatty acid on HR variability and R-R Interval. Methods: A 2 week randomized study was conducted. Participants are sub-elite weightlifters. All the subject was in pre-competition training. They have to perform 3 heavy sessions of 80%-85%, 85%-90%, and 90%-95% of the training load accordingly. Between the 2 weeks, one is considered the treated week and the other week consider a non-treated. In the treated week they provide recovery drinks immediately after the training which contained Flaxseed, Almonds, cashews, During Training, and Recovery HR variability and R-R interval were monitored through a GPS Heart rate monitor. Raisin. Results: There are no changes found between a treated and untreated week on Heart rate variability and R-R Intervals. Conclusions: Although Researcher told us Omega-3 helps to reduce HRV in the long-term study but in this investigation that is not found because the study was conducted over a short period