Extraction of the aerial parts of Synedrella nodiflora was done by continuous and exhaustive extraction of the powdered plant material using petroleum spirit (60-80oC), Chloroform, Ethyl acetate and methanol as solvents respectively. The extracts were obtained were concentrated in- vacuo at 40oC using rotary evaporator. General phytochemical screening of the aerial parts of Synedrella nodiflora Linn (Asterceae) revealed the presence of flavonoids, alkaloids, tannins steroids, glycoside and cardiac glycoside. The antimicrobial screening of the extracts showed significant zones of inhibition against 70% of the test organisms. The Minimum Inhibition Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) against various organisms were conducted. The antimicrobial analysis of the isolated components was also conducted. These prove the folkloric usage of the plant and also indicated a broad spectrum activity of the plant extracts.