Prickly Pads species of Opuntia ficus-indica, which naturally grows in different areas of the Middle East like Jordan and Saudi Arabia. Samples were collected and chemically prepared investigated to their phytochemical constituents using Inductively Coupled Plasma - Atomic Emission Spectroscopy (ICP-EAS) for determining twenty-two trace of heavy elements and analyzing the oils extracted for identifying thirty-two organic compounds by Gas Chromatography-Mass (GC-MS) Spectroscopy Detection (GC-MS). In this research study, results of mean concentrations for heavy metals were ranged from non-detectable to 277 ppm, and the extracted oil analysis was dominated by 2- furancarboxaldehyde, 2,5 -dihydroxy-3,6-dimethylhyroxy-1,4 propanal, formic acid, propionic acid, undecanoic acid, 1,2-bisphenyloxycarbonylhydrazine, 2,3-dihydroxy acetic acid, 2,2-dihydroxyacetic acid, 2-Ethoxyethyl acrylate, acetic acid, methyl acetoacetate, 2- (2 methoxyethoxymethyl) oxirane, ethane, 2-hydroxy-1,2-diphenylethanone, 2-Hydroxyethyl propanoate, Hydrazine, 4H-Pyran-4-one, 2,4,5-trimethyl-1,3-dioxolane, 5-hydroxymethyl-2- furaldehyde, 4-methyl-4-hepten-3-one, 1H-Pyrazole 1,3,5-trimethyl-1-butene and 2-chloro-3-methyl-cyclohexene as major and minor components were ranged from very low percentage to %.