Polyglutamic acid (PGA) is a natural biopolymer made up of periodical units of both L-glutamic acid, D-glutamic acid. This biopolymer is of special interest based on its biodegradability, non-dangerous and non-immunogenic properties, and thus it has been utilized effectively in many pharmaceutical applications. Among other novel applications, it has the likelihood to be utilized for protein crystallization, as a delicate tissue follower and a nonviral vector for safe quality conveyance. Therefore, PGA exhibited many therapeutic applications as anticancer agent, drug delivery, biological Glues, biological control agent, and in tissue Engineering. Investigations of PGA biosynthesis/production and learning of the catalysts and functional biological actions will additionally increase the range of its applications.