Abstract: Raman microscopy is an emerging tool in biomedicine. It provides a label-free and noninvasive analysis of biological samples. Due to its high biochemical specificity, Raman spectroscopy can be used to acquire spectral fingerprints that allow characterizing cell types and states. Confocal Raman microscopy enables measurements of individual cells in 2D-cell cultures and within 3D-tissues. The integration of simultaneous laser trapping features allows reliable Raman spectra acquisition even of motile bacteria or cells within the solution. This combined Raman-trapping microscopy (RTM) offers new opportunities in selective biochemical analysis and cell sorting.