The reproductive effect of pefloxacin (PF) and antioxidant vitamins C, E and garlic in wistar rats were evaluated. Fifty adult Albino wistar rats (120-180), of either sex were randomly selected into five study groups. Fifty rats were cohabited in matting ratio of 1:1 to give 5 study groups of 5 matting pairs per group. Reproductive parameters assessed were litter size, weight of offspring, morphological, organ weights. Control group was given placebo 0.5ml of normal saline while group II (pefloxacin 11.43mg/kg body weight), group III same dose of pefloxacin with 14.29mg/kg body weight of vitamin C supplement, group IV same dose of pefloxacin with 14.29mg/kg body weight of vitamin E supplement and group V pefloxacin with 4.28mg/kg body weight of garlic supplement in normal saline (vehicle) was administered orally by intubation to both male and female of groups 11 to V for 14 days. There was decrease in number of births (litter size) from 10 in the control to 2 in the PF treated and the PF treated and antioxidant supplemented group had higher litter size (8, 10 and 6) compared to PF treated only. Mean weight of off springs were significantly (P<0.05) higher in PF supplemented groups than PF treated only. The antioxidant properties of garlic, vitamins C and E particularly, their ability to ameliorate toxic effect of pefloxacin on affected reproductive organs were clearly depicted by the reversal of pefloxacin toxic effect on these organs and litter size.