Der Pharmacia Lettre
Ali Nazari, Javad Khodayar M, Maryam Salecheh, Mohsen Rezaei, Alireza Jahangiri
If a significant relationship is proven between toxic dose of acetaminophen on liver tissue and paraoxonase and arylesterase activity of paraoxonase (PON1) and profiles of lipids and serum lipoproteins, this enzyme can be used as a diagnostic biomarker in acute liver injuries, including damage caused by toxic drugs such as acetaminophen. At the beginning of the experiment, the rats were randomly assigned to 6 groups taking acetaminophen (0, 500, 1000, 2000, 3000 and 4000 mg/kg); then, activities of paraoxonase, arylesterase, serum malondialdehyde, liver tissue, ALT level, liver index and HDL/LDL were measured. Paraoxonase and arylesterase activity was lower in serum of groups receiving acetaminophen than the control group. Similarly, activity of HDL/ LDL was lower in serum of groups receiving acetaminophen than the control group; however, MDA activity increased in serum of groups receiving acetaminophen compared to the control group. Moreover, activities of ALT and liver index increased in groups receiving acetaminophen compared to controls. The results showed that tissue MDA was higher in groups receiving acetaminophen than the control group and tissue paraoxonase and tissue arylesterase do not follow any particular trend. In this study, no significant relationship was found in toxic dose of acetaminophen between intervention groups and controls and liver tissue, paraoxonase and arylesterase activity of paraoxonase (PON1) and HDL/LDL. Thus, this enzyme cannot be used as a diagnostic biomarker in acute liver injuries.