Standardization of siddha preparations is of utmost important task in establishing the active components of the drug responsible for its biological activity. The World Health Organization (WHO) guideline on evaluating the physiochemical property of a siddha formulation offers a greater value of Indian formulation in global market. Characterization of siddha formulation renders wide range of information in predicting the nature and structure of phytoconstituents which renders the actual therapeutic efficacy of the formulation. Even centuries before Indian systems of traditional medicines like siddha are the pioneers in developing nano drug delivery to the target site. The main aim of the present study is to prepare nano size formulation Singi Chenduram (SC) and also characterize the same by using sophisticated techniques like Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM). The results obtained from the SEM analysis shows that most of the particles present in SC are in nano and near nano size ranges from 71.32nm – 95.20nm.Further results obtained from the TEM study clearly projects that all the particles present in aggregates of SC formulation are within the size range of 50nm-200nm.This results shows that the formulation SC contains nano size particle which claims for its efficacy against the dreadful infectious disease like tuberculosis. In conclusion data’s retrieved from the current characterization study of SC confirms the size, shape and nature of nano-components of SC and hence this could be used as standards for evaluating quality and reproducibility of the Singi Chenduram in future.