The present study was taken up to assessment of antifungal activity of quaternary chitosan acrylic acid (Q CS-PAA) nano particles mordant dyed fabrics. The results indicate that the samples treated with Q CS-PAA exhibit higher inhibition activity than the untreated samples. The percentage of mycelium growth inhibition at higher concentration of 8oo ppm was varied from 68.06% on Fusarium solani to 64.09% on Penicillium decumbens. Findings of present study indicate that there are variation in tolerance to nano-QCS-PAA between different fungi Treatments with logwood dye in combination with alum and ferric chloride mordants inhibit fungal growth of both fungi. On the other hand, treatment with cochineal dye alone and in combination with mordants were not effective against the above mentioned fungi (0.00 mm zone of inhibition). The results showed an increase in inhibition % when dyeing cotton tissue was dyed with rhubarb dye by 6.95%, while opposite trend was occurred when the polyester fabric treated with rhubarb dye by 8.21%. Results clearly showed that treatment of textile with logwood dye only resulted in a decrease in efficiency of Q CS-PAA on the growth of fungus, as the zone of inhibition were dropped to 12.69, 20.65 and 8.69 % in polyester,, cotton and blend than non-dyed fabrics respectively. Polyester treated with Q CS-PAA + cochineal dye with alum and ferric mordants lead to increase inhibition zone by 11.11 and 35.48% respectively in comparison to treatment with Q CS-PAA + cochineal dye only. The treatment of Polyester/cotton blend by mordants lead to an increase by 6.95% . On the hand, opposite trend was observed with ferric mordant , as the zone of inhibition were dropped to 0.00 in polyester/ cotton blend treated with ferric .