Rapeseed is a new oilseed crop in Iran and its hectares has been currently expanding. Reproductive growth of the crop is exposed to water stress in many part of the country. In order to evaluation of yield, yield component and seed oil content of Brassica oilseed rape cultivars belong to Brassica napus, Brassica rapa and Brassica juncea species at stress and non stress locations with application of Pseudomonas strains , two experiment ( including stress and non stress fields) were carried out on 2010-2011 growing season at Agriculture and Natural Resources Research Station of Torogh, Mashhad. Experimental design was factorial based on Randomized Competently Block Design (RCBD) . Results showed that drought stress caused to reduction of yield , oil content and yield components. Also the negative forceful effect of water deficit on yield components was regarding pod numbers. Violently final yield decreased( 152.5% ) in stress location. There were considerable difference between stress and non stress locations regarding yield, oil content and all yield components. Except number of lateral branches , significant difference at 1% probability level were found among cultivars in studied characteristics .It was concluded that inoculation treatment had better effects than either no inoculation (control) or co-inoculation but interestingly co-inoculation with Pseudomonas flourescens plus Pseudomonas putida was placed at the lowest rating in comparison with individual inoculation or no inculcation at surveyed traits.