In the present study novel pyrimidine derivative of 4-(1H-indol-3-yl)-1, 6-diphenyl-5,6-dihydropyrimidin-2 (1H) one were synthesized and evaluated for their in vitro anti tuberculosis activity. A series of chalcones from indole-3-carbaldehyde and substituted aromatic ketone was synthesized and cyclo condensed with phenyl urea to give indoyl-pyrimidone derivatives. All synthesized compounds were characterized by IR spectroscopy and purification determined by melting point and TLC. Compounds were evaluated for their anti-tuberculosis activity against mycobacterium tuberculosis (H37RV Strain). Among the synthesized compounds IP-2 and IP-3 exhibited 6.25 µg/ml and found to be significant in activity when compared with standard drug (3.12 µg/ml).