Anthurium andreanum Linden plant species which belong to Araceae family has high commercial value. In this research, two varieties of A.andreanum have been micropropagated from calli by tissue culture techniques instead of classic production to increase the production. Plant regeneration of A.andraeanum varieties “Red Love” and “Pink Champion” have been achieved through adventitious shoot formation from calli. Leaf and petiol explants were harvested from mature certificated plants which were grown in the pots. The best combination for calli biomass production was obtained with combination of 0.1mg/L 2,4-D and 1.5mg/L BAP. Best shoot regeneration capacity was calculated as 86% per explant in the half strengthMS medium which was supplemented with 0.5 mg/L BAP for ‘Red Love’ variety. As a result of our research, in vitro optimization of “Red Love” and “Pink Champion” varieties have been realized in tissue culture conditions. Furthermore, micropropagation of Red Love variety was successfully achieved during our experiment.