A completely randomized design was conducted to evaluate the effect of egg powder application in pre-starter diet (0-7 d of age) of male broiler chickens. 320 male broiler chickens (ROSS 308) consumed different levels of egg powder (0, 2, 4 and 6 percent of diet) in their pre-starter diet from hatch until 7 d of age. Each treatment had 4 replicates with 20 male broiler chickens. Experimental sampling was carried out in 7 and 42 d of age. Results showed that relative weight of liver was not affected by egg powder application in pre-starter diet (p>0.05), while, increase in egg powder inclusion level of pre-starter diet led to gall bladder weight increase in both 7 and 42 d of age (p≤0.05). At 7 d of age, concentration of Ca ion reduced (p≤0.05) in serum of chickens which consumed egg powder in their pre-starter diet. Experimental treatments, however, had no effect on serum Ca concentration at the end of experiment (p>0.05). Cholesterol and triglyceride concentration in serum of chickens, which consumed egg powder in their pre-starter diet rose at 7 d of age (p≤0.05); the reverse trend was observed at 42 d of age though (p≤0.05). As a result of egg powder application at both 7 and 42 d of age, Serum concentration of total protein increased (p≤0.05) and malondialdehyde (MDA) decreased (p≤0.05). Result presented in this study showed that application of egg powder in pre-starter diet of broiler chicken significantly affected bird’s metabolism which is reflected by change in serum metabolites.