The purpose of this study the effects of Taurine supplementation for two weeks and three sessions of 90minutes of intense exercise on serum cytokines responsesinwell-trained soccer players. Therefore, Eighteen football players under 21 years of leaguewere selected and divided randomly to Taurine (TG=6), placebo (PG=6) and control (CG=6) groups.TG Daily supplements of 15 mg Taurine per kg of body weight and PG groups received the same amount of aspartame and both groups preformed three times a soccer specific exercise protocol. The CG group received no supplementation and follow-up was just your ordinary Program. Blood samples were taken in the sixth stage (48 h before Period, before and immediately after first & third exercise protocol and 48 h after the end of the Period) of the 5cc of the anterior forearm venous in sitting position. Then the samples to determine changes in cytokine (IL-6, TNF-α) in the central endocrine laboratory by special kitswere tested. The ANOVA with repeated measures and Tukey’s post hoc test with Spss.18 software was used to compare the means in each group. Results showed that the six-stage measure, significant differences (P=0/035) in levels of serum IL-6 has emerged and the difference between control group and the placebo group (P=0/049) has been observed. The serum TNF-levels no change significantly (P=0/937) in any group during the measurement. It appears that the three 90-minute soccer specificprotocol toenter stress on the inflammatory system in soccer players such pressure may again be repeated during the competitionseason. The other hand, results showed that Taurine supplementation before and during this period have anti-inflammatory effects and the noticeable changesto these cytokinesare prevented. Thus, short-term use of Taurine supplementation during pressure-filled week of competition and training to be advised in elitesoccer players.