By developing nanotechnology, researches about its destructive effects on organisms become important more and more. Titanium dioxide is used in production of various paints, cosmetics and so on. In this study, the effects of titanium dioxide nanoparticles on white blood cells of laboratory mice were investigated. Forty mature Syrian mice from Wistarrace were selected, and divided in four groups: placebo group, control group and two groups of 10 and 100 ppm doses of nanoparticles. Titanium dioxide nanoparticles were solved in water (20 nm) and mice were fed orally for 14 days. After this period blood samples were taken and white blood cells were counted. Titanium dioxide nanoparticles increased white blood cells and lymphocytes significantly whereas neutrophils were decreased severely. According to results, white blood cells were affected by nanoparticles. Therefore, titanium dioxide can affect also defense potential of blood.