Titanium dioxide is a nanoparticle which is used widely in order to control microorganisms and pathogens in health products and in a wide range of applications and commercial use. Current study was carried out to investigate the effects of titanium dioxide on reproductive hormones in female mice. Forty female mice were divided in to four groups: control, placebo, and two experimental groups. Placebo group did not receive anything except water and standard food. Experimental groups received 10 and 100 ppm of titanium dioxide in water. After 14 days, blood samples were taken and estrogen, progesterone, FSH and LH amounts were measured. Results showed that 10 and 100 ppm amounts reduced the amounts of FSH, LH, estradiol and progesterone significantly. Therefore, titanium dioxide can affect reproductive activities by changing pituitary gonadal axis and has negative effects on reproductive potential of female sex.