Investigation into the incidence of asymptomatic urinary tract infections was carried out using a total of 100 samples (50 early morning midstream urine and 50 high vaginal swab samples , respectively), from undergraduate female students of Imo State University and Federal University of Technology,Owerri. Isolation, characterization and identification of the microorganisms were done according to standard methods and reference manuals. A total of 48 organisms (48.0%) were obtained, which include: Escherichia coli (25.0%) Enterobacter aerogenes (12.5%), Pseudomonas aeruginosa (14.6%), Klebsiella pneumoniae (18.8%), Proteus mirabilis (4.1%), Staphylococcus aureus (20.8%) and Candida albicans (4.2%).The isolates showed variations in susceptibility to antibiotics used. This study has shown that asymptomatic bacteriuria is present in this seemingly healthy population of female students of the two Universities. There is need, therefore for routine checks for these infections and their earlier treatment, in order to avoid complications.