The purpose of this study was to examine the influence of vitamin E and C supplementation and strenuous aerobic training exercise on plasma cytokines in college male students. Twenty two non-active college male students (age: 24.86 ± 4.96 year, height: 173.14 ± 4.96cm, weight :68.18 ± 8.58kg and VO2max: 48.90 ± 5.91 mlÖ¾¹kgÖ¾¹min ) were randomly assigned to either an antioxidant supplementation(AS) or placebo(PLA) group. The subjects received 400 IU Vitamin E and 1000 mg Vitamin C (AS group) or 1400 mg cellulose combination (PLA group) with dinner per day in a two-blind design and performed a strenuous aerobic exercise training until exhaustion with %75 of VO2max, 5 days per week for a period of 4 weeks. Blood was collected pre and post exercise .The tests of aerobic power (Bruce test), body composition (skin fold) and blood TNF-α & IL-6 were measured by Elisa method before and after the intervention. Antioxidant Supplementation significantly decreased TNF-α & IL-6 in AS group compared to the PLA group (P≤0.05). It was concluded that in untrained humans vitamin E and C supplementations and strenuous aerobic training exercise decrease plasma cytokines.