Ginger herb is grown in many areas around the world it is used as medicinal herb. However, only a limited amount of research exists to support their efficacy. The aim of the present work is to demonstrate the antispasmodic effect of ginger (Zingiber officinale) on rat intestine in vitro. Methods Rats (150–200 g) were used throughout the experiment, Sacrificing was done by cervical dislocation The abdomens excised immediately, the jejunum Segments 2 cm long are cut mounted in a 10 ml tissue automatic organ bath containing Tyrode’s solution at 37◦C and aerated with carbogen (oxygen + 5 % carbon dioxide) gas, where one end is attached to the hook and the other is tied by a thread to the transducer. The transducers were connected to an amplifier to amplify the magnitude of contractions; these in turn were interpreted by a data acquisition system to obtain the final results by computer system. Results The results revealed that the effect of low doses of ginger on exogenous acetyl choline (ACh) induced contraction is spasmogenic as a dose of 0.2ml=20μg of ginger / tissue bath produced increase in the magnitude of ach induced contraction from 0.91 to 1.17 , while doses of 0.4 ml = 40μg of ginger/of tissue bath produced decrease in magnitude of ach induced contraction from 0.61 to 0.45 , and a dose of 0.8 ml=80μg of ginger / tissue bath produced decrease in magnitude of ach induced contraction from 0.85 to 0.11.