Using plants for treating diseases is followed since ages. Phytochemicals present in the plant have been proven to show various bioactivities like antibacterial, antifungal, antimicrobial, anticancer, antioxidant activities etc. In this study, Acacia Senegal was collected from Chennai, Tamil Nadu, India. The crude extracts were obtained using various solvents like ethanol, chloroform and petroleum ether. The obtained extracts were exploited for phytochemical analysis, and TLC bio autography analysis for antioxidants and antibacterial activity. The extracts which showed activity were fractionized using column chromatography and subjected for spot assay for antioxidant activity and antibacterial activity.