Research Article
Phylogenetic Analysis of Human Papilloma Virus Isolated from Women in
Abuja, Nigeria
Author(s): P.Y. Aondona*, F.A. Kuta, M. E. Abalaka and N. U. Adabara
Human Papilloma Virus (HPV) infection causes over 99% of cervical cancer and detection of HPV types might identify
women at risk of development or progression of cervical cancer. This study was aimed at determining the phylogenetic
relationship between the HPV strains isolated and identified. Cervical swabs were collected from the cervix
and DNA was obtained by extraction and then used to identify HPV types by PCR method using consensus primer
sets MY09/MY11 and GP5+/GP6+. A phylogenetic study was also carried out to determine the genetic association between
strains discovered using CLUSTALW on Unipro Ugene software. All HPV isolated in this study showed 80-99%
nucleotide identity with types related to the same species. The phylogenetic tree was constructed using 15 isolates
from this study against 45 reference strains selected from NCB.. Read More»
