Bacterial isolate Pseudomonas pseudoalcaligenes was isolated from the saline areas of Gujarat. This isolate showed efficient production of PGPR attributes like phosphate solubilization, Siderophore production and IAA production under salinity stress both in vivo and in vitro. Field studies revealed that they have the potential to increase vegetative growth of Jatropha curcas plant under salinity stress, and increase the soil fertility. To understand the mechanism of salt tolerance, proteome analysis of Pseudomonas was carried out using SDS PAGE. Salt stress proteins associated with cell membrane were analyzed by MALDITOF. This technique was used to investigate the regulation of gene product expression of P. pseudoalcaligenes grown under high osmolarity. Peptide mass fingerprinting and in silico investigation was used to identify proteins with altered expression. Among them one newly induced protein was assigned to protein with known function. Bioinformatics tools were then employed to identify the protein to understand the proteomics of salt stress in the bacteria. The protein showed high homology to the enzyme, ABC transporter, and iron.B12.siderophore. hemin, ATP-binding component.