A total of 18 samples made up of six samples from hospital and 12 samples from municipal solid waste dump soil in Benin City were collected for isolation of viable aerobic bacteria. The following aerobic bacteria in decreasing order of prevalence were isolated from municipal solid waste, aerobic spore bearers (83.33%), Escherichia Coli (50.00%), Staphyloccus aureus (14.67%), Klebsiella sp (8.33%). Samples from the hospital waste yielded Pseudomonas aeruginosa, Klebsiella sp, Bacillus substilis with a prevalence of (60.00%) each, Serratia sp,Staphylococcus aureus and Escherichia Coli (20.00%) each. Antibiotic susceptibility tests on isolates were performed using standard disc diffusion method. Overall, Ciprofloxacin was the most effective agent on the isolates followed by Augmentin and Gentamycin in hospital and municipal solid waste respectively.Almost all isolates were resistant to Cloxacillin and Amoxiallin.