The leachate samples from the Ekiti-State Government Destitute Centre used by the Ekiti State Waste Management Board [EKWMB] Ado-Ekiti were subjected to bacteriological, mineral and radioactive analyses. The bacteria were isolated using serial dilution procedure and pour plate method. Biochemical tests like catalase, citrate and so on were used to identify the isolated organisms. Atomic Absorption Spectrophotometry method was used to analyze the heavy metal contents in the leachate samples. Natural radioactivity content of soil samples from the dumpsite was also determined using a NaI(Tl)-based gamma counting system. The total bacteria and coliform counts ranged from 70.6 x 107 CFU/ml to 7.3 x 108 CFU/ml and 39.9 x 107 CFU/ml to 1.9 x 108 CFU/ml respectively. There is significant difference at P<0.05 between total bacteria and coliform count base on period of sample collection whereas there is no significance between total bacteria and coliform based on points of collection. The isolated bacteria and percentage occurrence includes; Escherichia spp (32%), Enterobacter spp (18%), Klebsiella spp (14%), Bacillus spp (12%), Enterococcus spp (9%), Salmonella spp (6%), Pseudomonas spp and Staphylococcus spp (4%). The mineral value ranged as follow; zinc (0.001mg/L–0.02mg/L), lead (0.001mg/L-0.002mg/L), copper (0.001mg/L–0.02mg/L), cobalt (0.001mg/L- 0.02mg/L) and in all samples mercury was not detected. Mean concentrations of 974±67, 35±3 and10±2 Bq kg-1 were obtained for 40K, 226Ra (238U) and 208Tl (232Th) respectively. The average absorbed dose rate and annual effective dose equivalent amounted to 63nGy h-1 and 0.08 mSv y-1 respectively. These values did not constitute any radiological burden to human population. All the organisms exhibited a high level of resistance to most of the antibiotics used. There is urgent need for awareness to be created about the present situation of the leachate to alert the communities living around the area on the need for treatment of the stream before they can be used for drinking and other domestic uses.