Trichoderma reesei was cultured to secret enzymes using orange mesocarp as subsrate.
Untreated orange mesocarp (non-alkali treated orange mesocarp washed with distilled water)
and orange mesocarp alkali-treated at 100 â°C, washed with water at pH of 6-7.When untreated
orange mesocarp and alkali-treated orange mesocarp was hydrolysed at varying temperatures of
30â° C,37 â°C, 40 â°C,50 â°C 55 â°C, appreciable glucose yield was found(2.13, 2.34, 1.71, 2.56,
2.35) mmol/L respectively for alkali –treated mesocarp, but the yield was lower when untreated
mesocarp was hydrolysed at 37 â°C(1.7mmol/L).Hence it was concluded for this particular
research that untreated orange mesocarp is as good a substrate for glucose production but the
optimum temperature for the alkali-treated mesocarp falls within 37-50 â°C.