Calcium carbonate (CaCO3) nanoparticles were successfully synthesized using calcium hydroxide (Ca(OH)2) and carbon dioxide gas by bubbling method. Resulting material characterized by powder X-ray diffraction (PXRD), FT-IR, UV-Visible, EDX and SEM. The crystal structure and particle size of nanoparticles determined by XRD using Debye Scherer formula. FTIR confirmed the formation of calcite, absorption bands at 719 cm-1, 873 cm-1, 1390 cm-1 corresponds to in-plane bending (υ4), out-of-plane bending (υ2) and asymmetric stretching (υ3) modes of carbonate group respectively. Morphology and elemental composition of CaCO3 NPs determined by SEM and EDX, respectively. The prepared nanoparticle further used for the fabrication of carbon paste electrode to determine the electrochemical oxidation of adenine and guanine residues in Fish sperm DNA. The results indicated that the oxidation enhanced peak currents for adenine and guanine residues of FsDNA, as compared with bare carbon paste electrode (BCPE).