The Interaction of 5-Bromouracil (5BU) and 5-Iodouracil (5IU) with bovine serum albumin
(BSA) were studied by spectroscopic method. Quenching of fluorescence of serum albumin by
these drugs was found to be a static quenching process. Based on the interaction studies carried
out the number of binding sites for drug on protein have been evaluated. The binding constant
(Ka) were 2.013 x 104 and 4.062 x 104 L mol-1 for 5BU and 5IU.