Development of biologically inspired experimental processes for the synthesis of nanoparticles is evolving into an important branch of nanotechnology. Metallic nanoparticles are traditionally synthesized by wet chemical synthesis techniques where the chemicals used are quite often toxic and flammable. The present study deals with cost effective and environment friendly given synthesis from 1mM AgNo3 solution through the leaf extract of Euphorbia hirta L, as reducing as well as capping agent. Nanoparticles were characterized using UV-VIS absorption spectroscopy’s. Green synthesized silver nanoparticles showed the antifungal activity against the Candida albicans, C.kefyr, A.niger.