Solanum pubescens Willd is an important medicinal plant which belongs to the family Solanaceae. It is widely used as folk medicine and treating for many diseases. Efficient in vitro regeneration of Solanum pubescens was achieved from node and Internode explants on MS medium with B5 vitamins and different concentrations and combinations of PGRs like BAP, GA3 and KIN. The maximum numbers of multiple shoots were achieved from nodal and internodal explants on 3.0 mg/l BAP+1.0mg/l GA3+0.5mg/l KIN (80.5%). The regenerated shoots were transferred in to half strength MS medium fortified with IBA for root induction. Rooted plantlets were successfully acclimatized. The current study showed efficient in vitro shoot regeneration capabilities of Solanum pubescens.