The deproteinised leaf juice (DPJ) of Lucerne ( Medicago sativa L.) was found to be suitable as a medium for the cultivation of Aspergillus niger. The fungus produced citric acid, when cultivated on DPJ. The production of citric acid increased with the supplementation of glucose to the DPJ. Thus the DPJ, left after preparation of leaf protein concentrate, was found suitable in microbial technology to produce citric acid and other useful microbial metabolites.
