The seeds of Gardenia aqualla fruit were analysed for nutritional and anti-nutritional composition using standard analytical methods. The results obtained were moisture content (49.00 %), ash (5.20 %), crude lipid (1.90 %), crude protein (4.46%), crude fibre (1.00 %), available Carbohydrate (87.64 %) and the energy value (384.70 kcal/100g). Elemental analyses show that potassium is the most abundant element in the seeds while chromium is the least (1.30mg/100g). Lead was also detected (0.43 mg/100g). The result of anti-nutritional evaluation indicate the presence of phytate (2.74 mg/100g), soluble oxalate (2.74mg/100g), saponin (5.10 mg/100g), nitrate (0.52mg/100g) and hydrocyanic acid (0.14 mg/100g). The antinutrients to nutrients ratio indicate the availability of some important minerals. The results indicate that the seed which is usually thrown away as waste can serve as a good source of some minerals and carbohydrate which if properly utilized would assist in combating the problem of malnutrition in the society.