Single crystals of pure 4-acetamidophenol (AMP) and 4-acetamidophenol (AMP) doped with tartaric acid were grown by single diffusion gel technique. These crystals are characterized by single crystal XRD, powder XRD, FT-IR, FT-Raman, UV-Visible spectroscopy, and SEM with EDX techniques to confirm the presence of tartaric acid in the 4-AMP crystal lattice. The single crystal and powder XRD studies reveal that the doping of tartaric acid disturbs the lattice of parent crystal. This lattice distortion affects the molecular composition of pure 4-AMP crystal. The FT-IR and FT-Raman spectral analysis confirms the presence of various functional groups present in the pure and doped 4-AMP crystals. The optical band gap and the transparency of the grown crystals are found by using the UV-Visible spectroscopy study. The surface morphology of pure and doped crystals have been examined by SEM analysis. The elemental analysis was done by EDX.