Total 185 patients of both sexes were included in study and further classified into 3 groups as hypertensive, Ischemic heart disease and cerebrovascular disease/stroke.The 60 healthy subjects in the control group were not on any kind of prescribed medication or dietary restrictions were included in the control group. In this study, we investigated first; the possible involvement of MDA and protein carbonyl as the end product of oxidative stress. and second we investigated serum lipid profiles (total, HDL and LDL cholesterol,trygliceride) to determine the relationship between these parameters in atherosclerosis. Serum triglycerides, total cholesterol, LDL cholesterol ,VLDL-cholesterol were significantly high in all groups than controls (p < 0.001) while HDL-cholesterol was found to be decreased significantly in all groups as compared to controls (p < 0.001). Total cholesterol, TC/HDL-C ratio, Triglycerides, LDLcholesterol, LDL-C/HDL-C ratio were higher in all three groups (p<0.001). HDL-C concentration was significantly lower in all three groups than controls (p<0.001). Higher ratio of TC/HDL-C, TG/HDL-C and LDL-C/HDL-C was observed in all three groups compared to controls. MDA is estimated as a marker of lipid peroxidation, levels were significantly increased in all groups than controls ( P <0.001). Carbonyl content is estimated as a marker of protein oxidation in red cell extract is found to be significantly increase in all groups when compared with normal ( P <0.001). The present study concludes the importance of assessing the lipid ratios even in a normal individual as it is one of the atherogenic factors for development of myocardial infarction and other coronary complications. The existing evidence supports the view that oxidative stress may play a crucial role in cardiac and vascular abnormalities in different types of cardiovascular diseases and that the antioxidant therapy may prove beneficial in combating these problems.