A Series of Immidazolines and Acetonitriles derivatives have been synthesized and tested for in vitro antibacterial activity on different microorganisms. Synthesis have been carried out following methodology in excellent isolated yields. The structure and purity of the original compounds were confirmed by IR, NMR, MASS spectroscopy and element analysis. All compounds were tested for antibacterial and antifungal activity against S.Aureus, E.aerogenes, B. coccus, P. aeruginosa. A. nige, C. albicans. These preliminary result indicate that some of compound are exhibiting good activity.