Helicobacter pylori multiply and cause infection in human gastric mucosal layer. New approaches have focused on using specific treatments, such as chicken antibody. The present study evaluated the potential use of immunoglobulin prepared from the egg yolk of chicken immunized with in the treatment of H. pylori infections. Between January and June 2011, a total of 107 H. pylori isolates were obtained from gastric biopsies of patients with upper gastrointestinal tract symptoms in endoscopy section at tertiary care hospitals in Coimbatore, South India. Genetic immunization of laying chicken with a plasmid expressing Helicobacter pylori UreB (large subunit of urease), IgY against UreB were obtained from egg yolks. These polyclonal and mono specific IgY antibodies are of higher-titer method such as Enzyme linked immunosorbent assay ELISA, Dot-blot and finally, urease neutralizing ability of the antibodies was evaluated in presence of the purified IgY. To our knowledge this is the first report describing generation of IgY antibodies directed against antigens of H. pylori by DNA-based immunization.