Four different types of explants from Paulownia elongata were used to assess the impact of plant tissue culture media with different concentrations of growth regulators and carbon sources on organogenesis and plant regeneration. When full leaf or half leaf explants with attached petioles were cultivated on either MS or B5 medium with 25 M thidiazuron, 10 M indole acetic acid, and 30 g L1 maltose as a carbon source, the best response to adventitious shoot proliferation was seen among the several treatments used. On a modified MS medium containing 5 M indole butyric acid, proliferated branches were rooted within five days, and plants were effectively acclimated and hardened for greenhouse transfer. Protocols for genetic transformation were improved by using the neomycin phosphotransferase II and green fluorescent protein genes. Using media that was tailored for shoot growth and rooted, Agrobacterium tumefaciens mediated transformation of half leaves with petiole explants yielded transgenic plants. PCR and RT-PCR were used to find the GFP transgene's insertion and expression.